| EPIDEMIOLOGY IN RELATION TO RISK AND POLICY: (WORK PACKAGE 3) |
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To quantify the sporulation, germination, infection, incubation period, latency, survival
and dispersal components of the epidemiology of European and American isolates of P. ramorum.
This will include defining the minimum, maximum and optimal temperature and moisture requirements for spore production
and germination in both European and American populations of P. ramorum (at least five isolates of each);
the potential for leaves of important tree and non-tree hosts (at least ten species of each) to produce inoculum
of the pathogen; the survival time of long-lived chlamydospore inoculum under different regimes of temperature and
moisture availability. The process of infection in wounded and unwounded hosts using containerised trees and excised material
will test trees (Quercus, Fagus, Abies and Taxus) and ornamentals (Viburnum, Rhododendron)
and the incubation period from infection to symptom expression will be defined in at least three model hosts (high, medium and low
susceptibility). The dispersal potential of the pathogen from discrete disease foci will be evaluated in nurseries and natural habitats,
by analysing foliage, soil and water samples using standard isolation methods and molecular diagnostics.
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